| 1. | Pcr primers design for gene chip
引物设计 |
| 2. | According to the nucleotide sequence of selected antigen epitope , pcr primers supplemented with the ecor i and sal i sites were designed
根据选出的抗原表位区的核酸序列设计pcr引物，并于引物末端添加ecor和sa11酶切位点。 |
| 3. | Their accession numbers in genbank respectively are af440390 , af440391 . primers serving as rt - pcr primers were synthesized based on the two cloned fragments
虽然这两段dna序列的相差很大，但是它们却编码着相同的氨基酸序列。 |
| 4. | To make the pcr primer that have adequacy enzyme sit flowed the sequence , ligate with the pgem - t easy after pcr . make sure the sequence by sequencing
经组织培养后，以pcr和rt - pcr法检测再生烟草，共得到转synnhap基因烟草4株， pbi121空载体浸染的对照烟草再生苗5株。 |
| 5. | Results show that pcr primer selection also plays decisive role in the methodology . two primer pairs considered universal to domain bacteria in previous reports did not work on our field sample
Dna提取和pcr的空白试验表明，本方法容易受到污染的影响，对实验材料和无菌操作的要求必须严格。 |
| 6. | Using the tpsl gene digested form the prokaryotic expressing vector as template , according to the published sequence , pcr primers of tpsl gene was designed , 1500bp fragment of tpsl was generated
以从t - vector中酶切得到的tps1为模板，根据已知序列设计pcr引物，扩增海藻糖- 6 -磷酸合成酶基因( tps1 ) 。 |
| 7. | Considering the essential doctrine of designing pcr primers , two regions in which all tigers share an unique hyplotype against other species were selected to design a pair of primers ( primer 1 , 2 ) to amplify a tiger cytb fragment specifically
发现在361 - 381位点之间和565 - 585位点之间，虎的dna碱基序列与其他猫科动物、鹿科动物有多个位点不同。 |
| 8. | The results indicated that the pcr primers designed could distinguish between marine bacteria and terrestrial bacteria , and could be applied in distinguishing the psychrophiles . sixteen strains which could produce cold - active protease and chitinase were screened by selective medium and
初步的分析表明，所设计的pcr引物能够较好地区分海洋性细菌和陆源性细菌，并且可以用于嗜冷海洋细菌的区分。 |
| 9. | 14 out of 25 strains of biocontrol agent streptomyces showed chitinolytic activity by biological assay . these 14 strains of biocontrol agent streptomyces were detected for chitinase gene using pcr primers derived from the conservative sequence of 17 cloned chitinase genes from streptomyces
此方法相对于几丁质酶的传统生物学检测，具有更好的准确性和灵敏性；而且与生物学检测相比，使用分子检测省时省力。 |
| 10. | Ii . molecular phylogeny in this paper , nucleotide sequences of the chloroplast gene trnl - f region was investigated first in the ferns , the pcr primers were chosen . in the test , 34 species in athyriaceae and 3 outgroup taxa were determined trril - f region sequences
介蔗属d尽口athyrium 、蛾眉蔗属lunathyrium 、假蹄盖蔽属athyriopsis 、单叶双盖蔗diplaziumsubsinuatum组成一支，在两种系统树中均得到100 %的支持率。 |
